Perfusion of isolated frogs heart with Ringer-tris solution.
نویسندگان
چکیده
منابع مشابه
پرفیوژن و جداسازی هپاتوسیت از کبد رات
Background and Purpose: Perfused rat liver and isolated hepatocytes are useful to study specific metabolism of this organ. Traditionally, mechanical, chemical and enzymatic methods have been used to isolate hepatocytes, and it appears that a mixed method is the preferred choice. Materials and Methods: The rat liver perfused in situe in three subsequent steps, 10 minutes with Krebs-Ringer s...
متن کاملFurther studies on perfusion of isolated frog's heart with modified Ringer solutions (glucose-Ringer, maltose-Ringer and lactose-Ringer).
The present series of experiments have served to elucidate unequivocally that the performance of isolated frog's heart improves considerably on reduction of sodium chloride to 60 mM/L in the perfusate, and that probably this improvement is not affected by the presence of disaccharides and glucose added to the perfusate so as to maintain isotonicity, although further work is required to corrobor...
متن کاملMorphological changes of heart muscle caused by successive perfusion with calcium-free and calcium-containing solutions (calcium paradox)
The phenomenon ‘calcium paradox’ has been found by increase of motional freedom of phospholipids and leakage Zimmerman and myself in 1966 [1]. At that time I was upon recalcification, resulting in loss of soluble cell interested in the effects of fatty acids and ketone bodies as constituents [4]. This paper mentions that cooling or energy sources for the beating heart, after studies on addition...
متن کاملA novel isolated dual perfusion/superfusion heart model for physiological and pharmacological evaluation of mammalian heart preparations
Abstract Introduction: Isolated perfused heart models such as perfusion and superfusion are commonly used for mammalian heart research. However, there are several fundamental limitations in the current techniques. In perfusion model, a suitable cannula is connected to the aorta and the perfusion is retrogradely performed. But, electrode displacement is a potential unwanted event resulted fr...
متن کاملAngiotensin-(1-7) potentiates the coronary vasodilatatory effect of bradykinin in the isolated rat heart.
It has been shown that angiotensin-(1-7) (Ang-(1-7)) infusion potentiates the bradykinin (BK)-induced hypotensive response in conscious rats. The present study was conducted to identify Ang-(1-7)-BK interactions in the isolated rat heart perfused according to the Langendorff technique. Hearts were excised and perfused through the aortic stump under a constant flow with Krebs-Ringer solution and...
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ورودعنوان ژورنال:
- Indian journal of physiology and pharmacology
دوره 10 4 شماره
صفحات -
تاریخ انتشار 1966